Fig. 4
From: Development of genome engineering technologies in cattle: from random to specific
Illustration depicting genome integration via the piggyBac (PB) transposon. The PB transposase recognizes the PB-long term repeat (LTR) sequences, cuts it, and inserts itself into a “TTAA” sequence in the host genome. The inset represent Hela cells with the PB- green (G)- and red (R)-fluorescent protein (FP) gene linked by 2A peptide sequences