Fig. 3

Activation of PPAR by NEFA in MAC-T cells is best represented by a quadratic model and is dose-dependent. PPAR activation measured using a 3xPPRE-TK-luciferase plasmid and normalized by co-transfected TK-renilla. Data are expressed as fold change over DMEM control (NEFA = 0 mmoL/l) by serum mixtures with different NEFA concentrations. The significance level of each parameter, as well as the R2 of the quadratic model are presented in the figure. Vertical bars indicate standard error