Fig. 10

Effects of PCA and Que on protein expression of pyroptosis signals after infection with ETEC K88 in IPEC-1 cells. Cells were pre-treated with 40 μmol/L PCA or 10 μmol/L Que for 24 h and then infected with or without 1 × 108 ETEC K88/mL for 2 h. a–e Protein concentration of pyroptosis signals. f Representative bands. Values are means ± SE, n = 6. a–dDifferent letters represent a significant difference, P < 0.05. IPEC-1, intestinal porcine epithelial cell 1; NLRP3, nod-like receptor protein 3; NLRC4, nod-like receptors family CARD domain-containing protein; ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D