Fig. 1

Histological organization of the anterior-mid intestine of gilthead seabream (Sparus aurata) fed experimental low fishmeal diets containing Debaryomyces hansenii (1.1%, 17.2 × 105 CFU; Yeast diet) (a) or devoid of the probiotic (Control diet) (b). Histochemical properties of mucins produced by intestinal goblet cells regarding their content on carboxylated and/or sulphated acidic groups from fish fed the Control diet (c) and the Yeast diet (d). Abbreviations: mc, mucous cells; bb, brush border. Staining: hematoxylin–eosin (a, b), Alcian Blue pH = 2.5 (c, d). Scale bar = 50 µm