Fig. 3

Microbial signatures for each sampling time point identified by LEfSe. A heatmap depicting the signature ASVs for each sampling time point identified by the LEfSe algorithm was drawn. The heat map shows the average relative abundances of ASVs on a log scale. The color of cells from blue to red corresponds to the relative abundance of ASVs from low to high. Ruminal fluids were collected on d 0, 28, 85 (S0, S28, S85) during the stocker phase and on d 0, 14, 28, 56, starting to add Pre-Ba (30 d before harvest) and at the end of finishing stage (F0, F14, F28, F56, Pre-Ba, and Final) during the finishing stage