Fig. 1

Effect of Lp. plantarum FRT4 on laying performance, liver morphology and biochemical parameters of laying hens. A The laying performance of laying hens in each group. The laying performance was corrected based on death rate. ADFI: Average daily feed intake; ADMEC: Average daily metabolic energy consumption. B Representative photographs of liver tissues of laying hens in each group. C Oil Red O staining observations of liver tissues of laying hens in each group (bar 100 μm). D Biochemical parameters of liver in each group. E Biochemical parameters of ovary in each group. The results were expressed as the mean ± SEM; n = 6 hens per group. * means the significant difference of HELP compared to CT group, and * means P < 0.05, ** means P < 0.01, *** means P < 0.001. # means the significant difference of FRT4L, FRT4M, and FRT4H compared to HELP group, and # means P < 0.05, ## means P < 0.01. CT: control group, hens fed with normal diet. HELP: model group, hens fed with high-energy low-protein diet. FRT4L, FRT4M, and FRT4H: experimental groups, hens fed with high-energy low-protein diet with 109 CFU/kg, 1010 CFU/kg, and 1011 CFU/kg Lp. plantarum FRT4, respectively