Fig. 2

Transcriptional characteristics and gene expression dynamics of F1s and POFs. A Venn diagram of differentially expressed gene (DEGs). Criteria for DEGs filtering were |\({\text{log}}_2(\mathrm{fold}\;\mathrm{change})\)|> 2 and Padj< 0.05. B Heatmap of all DEGs in four comparisons. Blue to red colors indicates the relative gene expression level from low to high, respectively. C–D Comparison and Pearson correlation analysis of fold change values in six DEGs between qRT-PCR and RNA-seq analysis, respectively. Gene Ontology (GO) terms of DEGs in CL_F1 vs. IL_F1 (E), CL_F1 vs. CL_POF1 (F), and IL_F1 vs. IL_POF1 (G). H Heatmap of DEGs involved in ovulation regulation