Fig. 3

Signaling pathways enriched in follicle ovulation by gene set enrichment analyses (GSEA). GSEA enrichment plots of significant pathways in follicle ovulation transition, the cytokine–cytokine receptor interaction pathway (A), lysosomes (C), the calcium signaling pathway (E), and apoptosis (G). The normalized enrichment score (NES), P-value, and FDR were determined using GSEA software and are indicated within each enrichment plot. B, D, F, and H Box plots showing fragments per kilobase of transcript per million mapped fragments (FPKM) of the key component genes in each pathway