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Table 3 Activities of enzymes for creatine synthesis in the tissues of hybrid striped bass1

From: Dietary glycine supplementation enhances syntheses of creatine and glutathione by tissues of hybrid striped bass (Morone saxatilis ♀ × Morone chrysops ♂) fed soybean meal-based diets

Enzyme

Tissue

0% glycine

1% Glycine

2% Glycine

P-value

Phase-I hybrid striped bass

 AGAT

Liver

1,870 ± 79

1,954 ± 81

1,966 ± 61

0.616

Kidney

3,030 ± 80c

3,572 ± 95b

3,843 ± 95a

 < 0.001

Skeletal muscle

5,164 ± 202c

6,431 ± 310b

7,170 ± 224a

 < 0.001

Proximal intestine

319 ± 16

326 ± 19

329 ± 15

0.911

 GAMT

Liver

2,107 ± 71b

2,225 ± 57ab

2,359 ± 67a

0.041

Kidney

3,052 ± 88

2,993 ± 143

3,036 ± 144

0.945

Skeletal muscle

7,234 ± 206b

8,875 ± 226a

9,402 ± 285a

 < 0.001

Proximal intestine

649 ± 30

659 ± 32

669 ± 19

0.878

Phase-II hybrid striped bass

 AGAT

Liver

1,880 ± 89

2,041 ± 79

2,071 ± 106

0.308

Head kidney

1,941 ± 73

1,935 ± 90

1,904 ± 55

0.931

Tail kidney

3,509 ± 101c

4,361 ± 186b

5,086 ± 180a

 < 0.001

Skeletal muscle

5,941 ± 161c

7,222 ± 181b

8,387 ± 105a

 < 0.001

Pancreas

3,643 ± 172b

4,728 ± 129a

4,727 ± 126a

 < 0.001

Proximal intestine

261 ± 13

269 ± 12

286 ± 18

0.477

 GAMT

Liver

2,066 ± 98b

2,240 ± 128a,b

2,482 ± 103a

0.046

Head kidney

ND

ND

ND

Tail kidney

4,030 ± 86

4,109 ± 159

4,043 ± 283

0.954

Skeletal muscle

8,159 ± 351b

9,640 ± 387a

10,517 ± 204a

 < 0.001

Pancreas

5,885 ± 207

5,910 ± 161

5,909 ± 157

0.994

Proximal intestine

ND

ND

ND

  1. AGAT Arginine:glycine amidinotransferase, GAMT Guanidinoacetate methyltransferase, ND Not detected
  2. 1Values, expressed as nmol/g of fresh tissue/30 min, are mean ± SEM, n = 8 fish per treatment group. Fish received dietary supplementation with 0%, 1%, or 2% glycine for 56 d. L-Alanine was used as the isonitrogenous control. Tissues were obtained from the fish at the end of the study
  3. a−cWithin a row, means not sharing the same superscript letter differ (P < 0.05)