Fig. 8
TW-7 may increase the level of histone lactylation of the PA zygotes derived from vitrified mouse MII oocytes via antioxidant pathways. A Representative images of DCHFDA staining in PA zygotes of Fresh, F + H2O2 and F + H2O2 + TW-7 groups. Scale bar, 50 μm. B The fluorescence intensity of ROS levels in PA zygotes of Fresh, F + H2O2, F + H2O2 + TW-7 groups. Fresh (n = 48), F + H2O2 (n = 47), F + H2O2 + TW-7 (n = 44). C Representative images of Pan Kla and H4K12la protein expression in PA zygotes of Fresh, F + H2O2, F + H2O2 + TW-7 groups. Scale bar, 50 μm. D The fluorescence intensity of LDHA and LDHB in PA zygotes of Fresh, F + H2O2, F + H2O2 + TW-7 groups. Number of PA zygotes involved in Pan Kla immunofluorescence staining: Fresh (n = 57), F + H2O2 (n = 54), F + H2O2 + TW-7 (n = 45). Number of PA zygotes involved in H4K12la immunofluorescence staining: Fresh (n = 39), F + H2O2 (n = 45), F + H2O2 + TW-7 (n = 40). E Representative images of EP300 and HDAC3 protein expression in PA zygotes of Fresh, F + H2O2, F + H2O2 + TW-7 groups. Scale bar, 50 μm. F The fluorescence intensity of EP300 and HDAC3 in PA zygotes of Fresh, F + H2O2, F + H2O2 + TW-7 groups. Number of PA zygotes involved in EP300 immunofluorescence staining: Fresh (n = 37), F + H2O2 (n = 31), F + H2O2 + TW-7 (n = 36). Number of PA zygotes involved in HDAC3 immunofluorescence staining: Fresh (n = 28), F + H2O2 (n = 33), F + H2O2 + TW-7 (n = 30). G Representative images of LDHA and LDHB protein expression in PA zygotes of Fresh, F + H2O2, F + H2O2 + TW-7 groups. Scale bar, 100 μm. H The fluorescence intensity of LDHA and LDHB in PA zygotes of Fresh, F + H2O2, F + H2O2 + TW-7 groups. Number of PA zygotes involved in LDHA immunofluorescence staining: Fresh (n = 36), F + H2O2 (n = 38), F + H2O2 + TW-7 (n = 38). Number of PA zygotes involved in LDHB immunofluorescence staining: Fresh (n = 51), F + H2O2 (n = 50), F + H2O2 + TW-7 (n = 52). Data in (B), (D), (F), and (H) were presented as mean percentage (mean ± SEM) of at least three independent experiments. *P < 0.05, **P < 0.01