Fig. 8

Quantitative visualisation (Sashimi plot) of differential alternative splicing events (DASs) statistically significant in the changes of percentage splicing inclusion (q < 0.05 and |ΔPSI| > 0.1) between visfatin-treated group (VIS1; purple) and control group (CTRL; red) samples. The numbers in each upper right corner of the tracks present the percentage of splicing inclusion (PSI) values. Splicing differentiations are covered by the number of reads mapped in the range of junction sites. The upper black tracks show the genomic localisation of splicing events. The plot was generated in ggsashimi Python script. Below of plots, we present validation results of chosen DASs using the PCR method. The images show the inclusion and skipping exon levels between VIS and the control group. Abbreviations: GPR173 - G protein-coupled receptor 173, MSL1 - MSL complex subunit 1, CFLAR - CASP8 and FADD like apoptosis regulator, MAPK6 - mitogen-activated protein kinase 6, PI4KA - phosphatidylinositol 4-kinase alpha