Fig. 8

Effects of autophagy inhibition on NLR family pyrin domain containing 3 (NLRP3) inflammasome-mediated signaling pathway. Bovine ruminal epithelial cells (BREC) were pre-treated with chloroquine (50 µmol/L) for 4 h, followed by treated with epigallocatechin-3-gallate (EGCG) (50 µmol/L) for 6 h and treated with lipopolysaccharide (LPS) (10 μg/mL) for an additional 6 h. A–D The production of inflammatory cytokines of tumor necrosis factor (TNF-α), IL-6, IL-10 and the caspase-1 activity. (E-M) Western blot analysis of the expression of NLRP3, pro-caspase-1, p62, LC3, ZO-1, Occludin, phosphorylated-nuclear factor kappa-B (p-NF-κB) p65, NF-κBp65, p-IκBα and IκBα. ^a–dValues without the same letters indicate a significant difference (P < 0.05)