Fig. 4

Iron deficiency and excessive iron induce oxidative stress detrimental to intestinal health. A Jejunum mucosa epithelium from each group were processed for morphological evaluation. B The relative villus height, crypt depth and villus/crypt were measured (scale bar = 100 μm). C mRNA expression of MUC1, MUC2, Occludin, ZO-1, Claudin-1, SOD1, SOD2, GPX4 and CAT, measured by RT-PCR in jejunum mucosa. D ROS levels were detected in jejunum. Data are presented as mean ± SEM and one-way ANOVA was performed. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001