Fig. 6

Iron levels affect jejunum and MLN immune cell populations. A Gating strategy for T cell flow cytometry in jejunum and MLN. Percentages of CD45⁺, CD3⁺, CD8⁺CD4⁻ and CD8⁻CD4⁺ detected by flow cytometry in jejunum (B) and MLN (C). D Gating strategy for macrophage flow cytometry in jejunum and MLN. Percentages of lymphocytes, CD45⁺, CD11b⁻, CD86⁺ and CD206⁺ detected by flow cytometry in jejunum (E) and MLN (F). Data are presented as mean ± SEM and student’s t-test was performed. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001