Dietary Zn proteinate with moderate chelation strength alleviates heat stress-induced intestinal barrier function damage by promoting expression of tight junction proteins via the A20/NF-κB p65/MMP-2 pathway in the jejunum of broilers

Table 2 Composition and nutrient levels of the broiler complete diets (as-fed basis)

Item	D 1–21	D 22–42
Ingredient, %
Corn	54.75	58.78
Soybean meal	36.31	32.56
Soybean oil	4.95	5.15
CaHPO₄^a	1.87	1.67
CaCO₃^a	1.20	1.09
NaCl^a	0.30	0.30
DL-Methionine^b	0.31	0.15
Micronutrients^c	0.31	0.20
Corn starch^d	0.00	0.10
Total	100.00	100.00
Nutrient levels, %
Metabolizable Energy, kcal/kg	3,045	3,102
Crude protein^f	21.44	20.08
Lysine^e	1.10	1.01
Methionine^e	0.61	0.44
L-Threonine^e	0.80	0.75
Tryptophan^e	0.24	0.22
Methionine + Cysteine^e	0.91	0.72
Ca^f	1.01	0.92
Non-phytate P^e	0.45	0.40
Zn, mg/kg^f	84.40	64.36

^aReagent grade
^bFeed grade
^cProvided per kilogram of diet for d 1 to 21: Vitamin A 12,000 IU, Vitamin D₃ 4,500 IU, Vitamin E 33 IU, Vitamin K₃ 3 mg, Vitamin B₁ (thiamin) 3 mg, Vitamin B₂ (riboflavin) 9.6 mg, Vitamin B₆ 4.5 mg, Vitamin B₁₂ 0.03 mg, Pantothenic acid calcium 15 mg, Niacin 54 mg; Folic acid 1.5 mg, Biotin 0.15 mg; Choline 700 mg, Cu (CuSO₄·5H₂O) 6 mg, Fe (FeSO₄·7H₂O) 40 mg, Zn (ZnSO₄·7H₂O) 60 mg, Mn (MnSO₄·H₂O) 110 mg, Se (Na₂SeO₃) 0.35 mg, I (Ca(IO₃)₂·H₂O) 0.35 mg; and for d 22 to 42: Vitamin A 8,000 IU, Vitamin D₃ 3,000 IU, Vitamin E 22 IU, Vitamin K₃ 2 mg, Vitamin B₁ (thiamin) 2 mg, Vitamin B₂ (riboflavin) 6.4 mg, Vitamin B₆ 3 mg, Vitamin B₁₂ 0.02 mg, Pantothenic acid calcium 10 mg, Niacin 36 mg; Folic acid 1.0 mg, Biotin 0.10 mg; Choline 500 mg, Cu (CuSO₄·5H₂O) 6 mg, Fe (FeSO₄·7H₂O) 30 mg, Zn (ZnSO₄·7H₂O) 40 mg, Mn (MnSO₄·H₂O) 80 mg, Se (Na₂SeO₃) 0.35 mg, I (Ca(IO₃)₂·H₂O) 0.35 mg
^dZnSO₄·7H₂O and Zn proteinate with moderate chelation strength added in place of the equivalent weight of corn starch to produce treatment diets as shown in Table 1
^eCalculated values
^fValues determined by analysis, and each value is based on triplicate determinations

ISSN: 2049-1891