Table 3 Primer sequences for real-time PCR amplification

Claudin-1

AY750897.1

F: 5'-CATACTCCTGGGTCTGGTTGGT-3'

100

R: 5'-GACAGCCATCCGCATCTTCT-3'

Occludin

NM_205128.1

F: 5'-GCAGATGTCCAGCGGTTACT-3'

150

R: 5'-ATGACGATGAGGAACCCACA-3'

ZO-1

XM_015278975

F: 5'-CTTCAGGTGTTTCTCTTCCTCCTC-3'

131

R: 5'-CTGTGGTTTCATGGCTGGATC-3'

JAMA

EF102433

F: 5'-CAGACCCCTACAAGAACCGC-3'

149

R: 5'-CACCTGGACGATGAGGTTGA-3'

A20

XR_005848478.2

F:5'-AAGAAGCCAGAAGGACAGAAGAAC-3'

83

R: 5'-CAGTGCTGCTCGCTGTAGATC-3'

NF-κB p65

NM_001012887.2

F: 5'-AAGATCTGGTGGTGTGCCTG-3'

137

R: 5'-AGTGGAACCTTTCGCGGATT -3'

MMP-2

NM_204420.3

F: 5'-CGATGCTGTCTACGAGTCCC-3'

96

R: 5'-TAGCCCCTATCCAGGTTGCT-3'

MMP-9

NM_204667.2

F: 5'-TCACGTACCGGGTGATGAAC-3'

288

R: 5'-TCACGTACCGGGTGATGAAC-3'

β-Actin

NM_205518.1

F: 5'- CAGCCATCTTTCTTGGGTAT-3'

169

R: 5'- CTGTGATCTCCTTCTGCATCC-3'

GAPDH

NM_204305.1

F: 5'-CTTTGGCATTGTGGAGGGTC-3'

128

R: 5'-ACGCTGGGATGATGTTCTGG-3'