Fig. 1
From: Seasonal influence on miRNA expression dynamics of extracellular vesicles in equine follicular fluid
EV characterization. A Transmission electron microscope (TEM) imaging shows EVs with typical structure (red arrow), scale bar = 100 nm. B Western blot analysis of EVs marker proteins CD81, FLOT-1, and TSG101 and cellular protein contamination indicator CYCS. C The NTA analysis reveals the size distribution (upper panel) and concentration (lower panel) of the isolated EVs, with the asterisk indicating a significant difference between groups. Data were analyzed and graphs were generated using GraphPad Prism version 8.4.2 (GraphPad; San Diego, CA, USA). Statistical differences were assessed between the mean values of more than two groups using One-way Analysis of Variance (ANOVA) followed by Tukey’s Multiple Comparisons tests. Data are presented as the Mean ± SEM of biological replicates. Statistical significance was identified at P ≤ 0.05