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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Dietary supplementation with N-acetyl-L-cysteine ameliorates hyperactivated ERK signaling in the endometrium that is linked to poor pregnancy outcomes following ovarian stimulation in pigs

Fig. 1

Ovarian stimulation triggers supraphysiological E2 levels and results in a decreased in pregnancy rate and litter size. A Schematic illustration of experimental design where gilts were treated with or without ovarian stimulation before FTAI. B–D The pregnancy rate (B), farrowing rate (C) and litter size (D) in gilts treated with (OS) or without (Control) ovarian stimulation. E and F The serum levels of E2 (E) and P4 (F) in control and OS groups. G Correlation between serum E2 levels and the number of piglets born, each dot represents a gilt. H Immunohistochemical analysis of PCNA in endometrium from gilts on d 12 of pregnancy in control and OS groups. Scale bar: 20 μm. I Western blotting analysis of PCNA in endometrium from gilts on d 12 of pregnancy in control and OS groups (n = 3). The upper panel shows the quantification of PCNA level. Data are presented as the mean ± SEM. n value represents the number of gilts. *P < 0.05, **P < 0.01. OS, ovarian stimulation; PMSG, pregnant mare serum gonadotrophin; GnRH, gonadotropin-releasing hormone; AI, artificial insemination

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891