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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Dietary supplementation with N-acetyl-L-cysteine ameliorates hyperactivated ERK signaling in the endometrium that is linked to poor pregnancy outcomes following ovarian stimulation in pigs

Fig. 3

The high level of E2 hyperactivates FGF-FGFR-ERK signaling cascade. A Relative mRNA expression levels of FGFs and their receptors in endometrial explants treated with or without the high level of E2 (n = 5). B Immunohistochemical analysis of FGF7 in endometrial explants treated with or without the high level of E2. Scale bar: 20 μm. C Western blotting analysis of FGF7 level in endometrial explants treated with or without the high level of E2 (n = 7). The upper panel shows the quantification of FGF7 protein level. D and E Western blotting analysis of phosphorylation levels of FGFR (D) and ERK (E) in endometrial explants treated with or without the high level of E2 (n = 7). The upper panel shows the quantification of pFGFR and pERK levels. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. E2, estradiol; FGF, fibroblast growth factor; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891