Fig. 5

The effects of IAld on histology and gut barrier function in the small intestine of weaned piglets. a The ratio of small intestinal weight to length. b The small intestinal index. c Representative H&E staining of small intestinal tissue sections from the CON and IAld groups of piglets (Scale bars, 200 μm). d The villus area. e Representative images of CON and IAld groups of piglets immunostained with an anti-Villin antibody (red) and counterstained with DAPI (blue) (Scale bars, 200 μm) in the small intestine. f Mean fluorescence intensity of Villin in the small intestine. g Representative images of CON and IAld groups of piglets immunostained with an anti-Mucin2 antibody (red) and counterstained with DAPI (blue) (Scale bars, 200 μm) in the small intestine. h Mucin2-positive cell per villi of the small intestine. i Relative mRNA expression of MUC2. j Immunoblot analysis of Occludin and Claudin1 in the small intestine (n = 4). k Relative protein expression levels of Ocludin and Claudin1 in the small intestine (n = 4). Data are expressed as the mean ± SEM. (a–i) n = 8. ^*P < 0.05