Fig. 6

The effects of IAld on intestinal epithelial cell proliferation and differentiation in the small intestine of weaned piglets. a Representative images of CON and IAld groups of piglets immunostained with an anti-Olfm4 antibody (red) and counterstained with DAPI (blue) (Scale bars, 200 μm) in the small intestine. b Mean fluorescence intensity of Olfm4 in the small intestine. c Representative images of CON and IAld groups of piglets immunostained with an anti-KRT20 antibody (red) and counterstained with DAPI (blue) (Scale bars, 200 μm) in the small intestine. d Mean fluorescence intensity of KRT20 in the small intestine. e The relative mRNA expression of Olfm4, Lgr5, PCNA, and LYZ in the small intestine. f The relative mRNA expression of AHR and CYP1A1 in the small intestine. g The relative mRNA expression of β-catenin, c-Myc, and Cyclin D1 in the small intestine. h Immunoblot analysis of Lgr5, active β-catenin, β-catenin, and PCNA in the small intestine (n = 4). i Relative protein expression levels of Lgr5 and PCNA in the small intestine. j The ratio of the relative protein expression levels of active β-catenin to β-catenin in the small intestine. Data are expressed as the mean ± SEM. (a–g) n = 8. ^*P < 0.05