Fig. 5

Effects of Nec-1 on protein expression of necroptosis signals in liver of piglets after DON gavage. Piglets were given a gavage with 2 mg/kg BW DON or an equal volume of normal saline after intraperitoneal injection of 0.5 mg/kg BW Nec-1 or an equal volume of 5% dimethylsulfoxide (DMSO). Pigs were euthanized at 6 h after DON or saline gavage. A–I Protein expression of necroptosis signals. J Representative bands. Values are means ± SE, n = 6. ^a,bValues without a common letter differ significantly (P < 0.05). DON, Deoxynivalenol; p-MLKL, Phosphorylated mixed lineage kinase domain-like protein; p-RIP1, Phosphorylated receptor interacting protein kinase 1; p-RIP3, Phosphorylated receptor interacting protein kinase 3; t-MLKL, Total mixed lineage kinase-like protein; t-RIP1, Total receptor interacting protein kinase 1; t-RIP3, Total receptor interacting protein kinase 3