Fig. 3

Effects of different concentrations of DON on lysosomes in IPEC-J2 cells detected by the Lyso-Tracker Red (mean ± SD, n = 3). Typical fluorescent staining of lysosomes at DON concentrations of (A) 0, (B) 1,500, (C) 2,000, and (D) 2,500 ng/mL. Scale bar is 5 μm. E Red fluorescence intensity of lysosomes. Significance was compared with the control group, and ** indicates P < 0.01