Fig. 6

CGA alleviates DON-induced pyroptosis in IPEC-J2 cells (mean ± SD, n = 6). A and B Typical TUNEL dying photograph and fluorescence intensity (mean ± SD, n = 6). Scale bar is 100 μm. C mRNA level of GSDMD. D and E Extracellular contents of Ca2+ and K+. F and G Enzyme activity of caspase-1 and caspase-4. ns indicates P > 0.05. Compared with the control group, ## and # represent P < 0.01 and P < 0.05 respectively, and compared with the other group, ** and * represent P < 0.01 and P < 0.05 respectively. GSDMD: gasdermin D