Fig. 6

Alpha diversity of the cecal microbiota, measured using the Shannon and Simpson indexes, in high (HHW) and low (LHW) hatch weight chickens on d 14 (A and B) and d 42 (C and D) that had received 3 levels of sodium butyrate in ovo (SB1: 0.1%, SB3: 0.3%, SB5: 0.5%) or 0.9% NaCl (control) in ovo. Two-way ANOVA was applied to the alpha diversity metrics (n = 6 birds/group) and P values are indicated by different letters corresponding to P (HW), P (SB), and P (HW×SB). a–cValues with different letters indicate statistical significance at P < 0.05