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Table 1 Effects of in ovo sodium butyrate administration on the body weight and average daily gain of broiler chickens with different hatch weights

From: In ovo sodium butyrate administration differentially impacts growth performance, intestinal barrier function, immune response, and gut microbiota characteristics in low and high hatch-weight broilers

Item1

Body weight, g

Average daily gain, g/d

1 d

14 d

35 d

42 d

0–14 d

15–35 d

36–42 d

0–42 d

HW

SB

        

HHW

Control

55.18

399

1,917ab

2,418b

24.85bc

71.96bc

72.73c

56.87bc

 

SB1

55.08

411

1,891ab

2,365bc

25.78bc

70.45c

67.45d

54.93cd

 

SB3

55.06

397

2,028ab

2,571ab

24.74c

76.15a

75.92bc

59.42b

 

SB5

55.20

335

1,668c

2,282bc

20.34d

63.50d

84.60a

54.91cd

LHW

Control

45.12

377

1,723bc

2,201c

24.01c

64.80d

67.87d

51.80d

 

SB1

45.48

441

1,944ab

2,499ab

28.55a

71.26bc

79.05b

59.23b

 

SB3

46.05

416

2,050a

2,651a

26.77ab

77.48a

87.01a

63.36a

 

SB5

45.78

379

1,912ab

2,342bc

24.10c

72.67b

61.93e

53.68cd

SD

 

2.301

54.5

221.3

238.7

3.112

4.831

8.434

3.773

Main effects

HW

 HHW

 

55.13a

386

1,876

2,409

23.93b

70.52b

75.18a

56.53

 LHW

 

45.61b

403

1,907

2,423

25.86a

71.55a

73.97b

57.02

SB

 Control

 

50.15

388

1,820b

2,310c

24.43c

68.38c

70.30c

54.34c

 SB1

 

50.28

426

1,918ab

2,432b

27.17a

70.86b

73.25b

57.08b

 SB3

 

50.56

408

2,039a

2,611a

25.76b

76.82a

81.47a

61.39a

 SB5

 

50.49

357

1,790b

2,312c

22.22d

68.09c

73.27b

54.30c

P value

 HW

 

< 0.001

0.087

0.576

0.668

0.079

0.029

0.027

0.256

 SB

 

0.4191

0.108

< 0.001

0.025

0.023

< 0.001

< 0.001

< 0.001

 HW × SB

 

0.419

0.061

0.029

0.045

< 0.001

< 0.001

< 0.001

< 0.001

  1. 1HW Hatch weight, SB Sodium butyrate inclusion level. Control: HHW or LHW chicks from eggs injected with 0.2 mL of 0.9% NaCl. SB1, SB3, SB5: HHW or LHW chicks from eggs injected with 0.2 mL of 0.1%, 0.3%, or 0.5% SB, respectively
  2. Body weight was recorded from individual birds, while a pen was considered the experimental unit for average daily gain (n = 6 pens/group). The data are presented as the mean and pooled standard deviation (SD)
  3. a–dValues with different superscripts in a column indicate statistical significance at P < 0.05 (two-way ANOVA followed by Tukey’s HSD test)