Fig. 2

Volcano plot showing the total number of differentially (DEG) and non-differentially (Not DEG) expressed genes in the yolk sac (YS) of broiler chicken embryos at embryonic day 19.5, after in ovo delivery of carvacrol at embryonic day 17.5 compared to the control (saline injected) group. Transcriptomes of 11 samples were used (control; n = 5, carvacrol; n = 6). Every dot represents one gene. In total, 10,901 genes were found expressed in the YS, from which 442 were DEG (P < 0.05, logFC < −0.5 or logFC > 0.5) between the 2 treatments (268 upregulated and 174 downregulated in the carvacrol group compared to the control)