Fig. 1

Fatty acid and amino acid profiles in breast muscle of spent hens (n = 8). The OPLS-DA score plots (A), heatmap of differential fatty acids (B), and enrichment of the metabolic pathways (C) for the CHF vs. CON groups. The OPLS-DA score plots (D) and heatmap of differential amino acids (E) for the CHF vs. CON groups. CON group, Conventional diet; CHF group, Conventional diet with the addition of 1% Chinese herbal formula; OPLS-DA, Orthogonal projections to latent structures-discriminant analysis