Fig. 1

Identification, differential genes and sequencing analysis of extracellular vesicles (EVs) from 3 sources of bovine milk. A Transmission electron microscopy observation of EVs. B Measurement of EV particle diameter of bovine colostrum, mature milk and mastitis milk (defined as C-EVs, M-EVs and CM-EVs, respectively). C Detection of EV markers by Western blot. D PCA based on DESeq2. E Analysis of miRNAs in CM-EVs vs. C-EVs based on volcano maps. F Analysis of miRNAs in M-EVs vs. C-EVs based on volcano maps. G Analysis of miRNAs in M-EVs vs. CM-EVs based on volcano maps. H KEGG top20 enrichment pathway analysis of CM-EVs vs. C-EVs. I KEGG top20 enrichment pathway analysis of M-EVs vs. C-EVs. J KEGG top20 enrichment pathway analysis of M-EVs vs. CM-EVs