Fig. 3

Characterization of circANKRD17 in adipocytes. A Circular structure diagram and Sanger sequencing results for circANKRD17. B Polymerase chain reaction (PCR) and agarose gel electrophoresis analysis of complementary DNA (cDNA) and genomic DNA (gDNA) using a double-primer method. C and D Analysis of circANKRD17 using RNase R digestion and actinomycin D treatment. circANKRD17 indicates a fragment with a junction sequence amplified by divergent primers, while linear ANKRD17 refers to a linear fragment amplified by convergent primers. E Distribution of circANKRD17 gene transcripts in 3T3-L1 cells, with U6 serving as a nuclear control gene and ACTB as a cytoplasmic control gene. F Localization of circANKRD17 observed via fluorescence in situ hybridization (FISH). Cy3-labeled circRNA appears red, while DAPI-stained nuclei appear blue. Scale bar = 10 μm