Fig. 1

Histological and transcriptional remodeling during whitening of the pAT and oAT in goats. A Representative H&E and UCP1 immunohistochemical stained images of adipose tissue depots from dairy goats at different ages (n = 3). Scale bars represent 50 μm. “D” refers to “day old” and “M” refers to “month old”. B Principal component analysis (PCA) plot of pAT and oAT at different ages measured by using bulk RNA-seq. C Average log2 expression level of UCP1 in goat pAT and oAT at the indicated age from bulk RNA-seq. FPKM, fragments per kilobase of transcript per million mapped reads. D The brown adipocyte content of pAT and oAT at different ages was calculated by the BATLAS web tool. E and F Heatmaps displaying k-means clustering of differential expression genes (DEGs) in pAT (E) and oAT (F) of dairy goats (left panel). The pathways enriched for each cluster were labeled in the right panel. **P < 0.05, ***P < 0.01 by one-way ANOVA followed by Bonferroni’s multiple comparisons