Fig. 3
From: Ferroptosis emerges as the predominant form of regulated cell death in goat sperm cryopreservation
Effects of ferroptosis and apoptosis inhibitors on sperm plasma membrane and acrosome integrity and RCD proportions of frozen-thawed goat sperm. A Flow cytometric analysis of plasma membrane integrity of frozen-thawed sperm treated with RCD inhibitors prior to freezing, with representative data from each group shown. The gated portion represents cells with intact plasma membranes. B Statistical graph of plasma membrane integrity for each experimental group in panel A. C Flow cytometric analysis of acrosome integrity of frozen-thawed sperm treated with RCD inhibitors, with representative data for each group shown. Quadrant analysis: Q1 represents cells with intact acrosomes and damaged plasma membranes (FITC − , PI +), Q2 represents cells with damaged acrosomes and damaged plasma membranes (FITC + , PI +), Q3 represents apoptotic cells with intact plasma membranes (FITC + , PI −), and Q4 represents cells with intact acrosomes and intact plasma membranes (FITC − , PI −). D Statistical graph of Q1 + Q4 for each experimental group in panel C. E Flow cytometric results show the occurrence of RCD in frozen-thawed sperm treated with RCD inhibitors, with representative data for each group. Quadrant analysis: Q1 may represent cell debris or cells undergoing apoptotic cell death (APC − , PI +), Q2 represents cells undergoing RCD (APC + , PI +), Q3 represents early apoptotic cells (APC + , PI −), and Q4 represents live cells (APC − , PI −). F Statistical graph of Q2 + Q3 for each experimental group in panel E, indicating the overall occurrence of RCD. G Statistical graph of Q3 for each experimental group in panel E, representing the early apoptotic cell population