Fig. 4
From: Ferroptosis emerges as the predominant form of regulated cell death in goat sperm cryopreservation
Effects of different regulated cell death (RCD) inhibitors on the ROS levels, lipid peroxidation, and Fe2+ levels of frozen-thawed goat sperm. A Flow cytometric results for ROS detection in frozen-thawed sperm treated with RCD inhibitors, displaying representative data for each group. B Statistical graph of the average dichlorofluorescein (DCF) fluorescence intensity for each experimental group in panel A, indicating ROS levels. C Flow cytometric results for lipid peroxidation detection in frozen-thawed sperm treated with RCD inhibitors, displaying representative data for each group. D Statistical graph of the average oxC11-BODIPY fluorescence intensity for each experimental group in panel C, representing lipid peroxidation levels. E Flow cytometric results of Fe2+ levels in frozen-thawed sperm treated with RCD inhibitors, with representative data displayed for each group. F Statistical graph of the average FerrOrange fluorescence intensity, representing Fe2+ levels, for each experimental group in panel E