Fig. 5

Immunofluorescence localization of MyHCI to detect primary skeletal muscle fibers and MyHCIIa to detect secondary skeletal muscle fibers. Biceps-femoris sections from replicate 1 were incubated overnight at 4 °C with primary antibodies at a 1:500 dilution for myosin heavy chain Type 1 to determine primary muscle fibers, and myosin heavy chain Type IIa to determine secondary muscle fibers. Five non-overlapping images were taken, then primary and secondary muscle fibers were counted blind to treatment by one individual. There was no significant (P > 0.05) treatment effect on primary or secondary muscle fiber counts for fetuses from either d 60 or d 90 of gestation. Values are mean \(\pm\) SEM (n = 24 CON d 60 fetuses, n = 30 Cr d 60 fetuses, n = 24 CON d 90 fetuses, n = 28 Cr d 90 fetuses)