Fig. 6

Representative images of candidate proteins expressed in endometria and fetal biceps-femoris muscle determined using Western blot analyses. Endometrial and fetal biceps-femoris samples from replicate 1 used for Western blot analyses. Primary antibodies used were rabbit polyclonal antibody CKMT1A (15346-1-AP, Protein Tech, Rosemont, IL, USA), CKMT2 (ab121450, Abcam, Cambridge, UK); CKMM (ab151465, Abcam); CKB (ab92452, Abcam); and GAMT (ab126736, Abcam), and all primary antibodies used at 1:500 dilution. Results of quantification of GAMT, CKB, CKM, CKMT1, and CKMT2 in endometria revealed no effects of treatment (P > 0.05). However, there was an effect of day of gestation with endometrial samples from d 90 of gestation having greater expression of CKMT1 than for samples from d 60 of gestation (P < 0.0001), values are mean ± SEM (n = 4 CON d 60 gilts, n = 5 Cr d 60 gilts, n = 4 gilts CON d 90 gilts, and n = 5 Cr d 90 gilts). There were no treatment effects on the expression of GAMT, CKB, CKMT2, and CKM proteins in fetal biceps-femoris muscle (P > 0.05). CKMT1 was not detectable in fetal biceps-femoris muscle (n = 1 CON d 60 fetuses, n = 1 Cr d 60 fetuses, n = 16 gilts CON d 90 fetuses, and n = 20 Cr d 90 fetuses)