Fig. 4

Eugenol alleviates Salmonella enteritidis-induced impairment of ISCs proliferation and differentiation. A–F Jejunal IF staining of keratin 20 (KRT20), SOX9, and C-caspase-3 (100 × magnification, scale bar = 200 μm). G and H Jejunal tissue protein levels of KRT20 and Villin. I and J Jejunal tissue protein levels of Lgr5, PCNA, C-caspase-3. K and L Jejunal crypt protein levels of KRT20 and Villin. M and N Jejunal crypt protein levels of Lgr5, PCNA, C-caspase-3. Data are shown as mean ± SEM (n = 3). Differences between groups were indicated by different lowercase letters (P < 0.05)