Fig. 2

ABX treatment resulted in the enrichment of Lactobacillus in the colonic microbiota of the piglets. A ACE, Chao1, Shannon and Simpson indices in α-diversity analysis (n = 6). B Principal Coordinates Analysis (PCoA) of β diversity. C ANOSIM based on Bray-Curtis distance (n = 6). D Relative abundance of microbiota at genus level. E The relative abundance of Lactobacillus and levels of secondary bile acid biosynthesis (KEGG enrichment analysis) in colonic microbiota (n = 6). F The copy number of total bacteria and Lactobacillus in colonic contents (n = 6). Statistical analysis: one-way ANOVA and Tukey’s post-test for the comparison of four groups (^****P < 0.0001; ^**P < 0.01; ^*P < 0.05)