Fig. 4

AFB1 enhances the expression of hepatic CYP8B1 and modifies bile acid synthesis in piglets. A Expression levels of hepatic CYP8B1, CYP27A1, CYP7A1 and CYP7B1 as determined by RNA-seq (n = 5 or 6). B Relative mRNA expression of hepatic CYP8B1 measured by qPCR (n = 6). C Heatmap showing the concentration of different BAs in jejunal contents. D Proportion and concentration of 12α-OH BAs in jejunal contents (n = 6). E The concentration of total bile acids in jejunal contents (n = 6). F Concentration of TC, TG and FFA in the colonic contents (n = 6). Statistical analysis: one-way ANOVA and Tukey’s post-test for the comparison of four groups (^**P < 0.01; ^*P < 0.05)