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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Effects of hypoxia stress on the milk synthesis in bovine mammary epithelial cells

Fig. 2

Effects of hypoxia on milk synthesis in BMECs. A Immunoblots of β-casein (CSN2) and κ-casein (CSN3) in BMECs after exposure to hypoxia for 0, 6, or 24 h (n = 3). β-actin was used as the loading control. B and C Quantitative analyses of the blot density in A. D and E The triglyceride content (n = 3) (D) and glucose uptake (n = 3) (E) in BMECs after exposure to hypoxia for 0, 6, or 24 h. F Quantitative analysis of the fluorescence stained in G. G The fluorescence staining of lipid droplets in BMECs after exposure to hypoxia for 0, 6, or 24 h. Blue: DAPI; Green: Bodipy. Data with error bars represent mean ± SEM. Statistical significance (P < 0.05) marked by different lowercase letters in individual bars was determined by ANOVA with Tukey’s multiple comparison test

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