Fig. 3
From: Effects of hypoxia stress on the milk synthesis in bovine mammary epithelial cells
Effects of hypoxia on protein expressions of milk fat and protein synthesis regulators in BMECs. A Immunoblots of the phosphorylated (P) forms and total levels of the mTOR signaling proteins in BMECs after exposure to hypoxia for 0, 6, or 24 h (n = 3). B–D Quantitative analyses of the blot density of the phosphorylated protein abundance relative to total protein abundance in A. E Immunoblots of FASN, PPAR-γ, and FABP3 in BMECs after exposure to hypoxia for 0, 6, or 24 h (n = 3). F–H Quantitative analyses of the blot density in E. For immunoblot, β-actin was used as the loading control. Data with error bars represent mean ± SEM. Statistical significance (P < 0.05) marked by different lowercase letters on each bar was determined by ANOVA with Tukey’s multiple comparison test