Fig. 7
From: Effects of hypoxia stress on the milk synthesis in bovine mammary epithelial cells
Effects of HIF-1α knockdown on milk synthesis in BMECs. A Immunoblots of HIF-1α , β-casein (CSN2), and κ-casein (CSN3) in BMECs under 24 h hypoxia and transfected with either the control (NC) siRNA or HIF-1α siRNA (n = 3). B–D Quantitative analyses of the blot densities in A. E and F The triglyceride content (n = 3) (E) and glucose uptake (n = 3) (F) in BMECs under 24 h hypoxia and transfected with either NC siRNA or HIF-1α siRNA. G Immunoblots of FASN, PPAR-γ, and FABP3 in BMECs under 24 h hypoxia and transfected with either NC siRNA or HIF-1α siRNA (n = 3). H–J Quantitative analyses of the blot densities in G. For immunoblots, β-actin was used as the loading control. Data with error bars represent mean ± SEM. Statistical significance (P < 0.05) marked by different lowercase letters on individual bars was determined by two-tailed unpaired t-test