Caffeic acid and chlorogenic acid mediate the ADPN-AMPK-PPARα pathway to improve fatty liver and production performance in laying hens

Table 1 Composition and nutrient contents of basal diet

Ingredient, %	Content	Calculated nutrition levels^c, %	Content
Corn (8.5% CP)	62.2	Crude protein	15.63
Soybean meal (46% CP)	23.2	Metabolizable energy, kcal/kg	2,635
Wheat bran	3.796	Calcium	3.84
Limestone	9	Total phosphorus	0.48
CaHPO₄	0.85	Available phosphorus + phytase	0.32
Zeolite powder	0.169	Lysine	0.79
Salt	0.3	Methionine	0.39
Choline chloride (50%)	0.1	Threonine	0.59
DL-Methionine (99%)	0.14	Tryptophan	0.18
Phytase (≥ 10,000 U/g)	0.02	Methionine + cystine	0.66
Mineral premix^a	0.025
Vitamin premix^b	0.2
Total	100

^aThe mineral premix provided the following per kg of diets: Mn 54 g, Fe 36 g, Cu 5 g, I 0.8 g, Zn 45 g, Se 0.18 g
^bThe vitamin premix provided the following per kg of diets: vitamin A 38,000,000 IU, vitamin D₃ 15,000,000 IU, vitamin E 60 g, vitamin K₃ 7 g, vitamin B₁ 6 g, vitamin B₂ 30 g, vitamin B₆ 15 g, vitamin B₁₂ 0.05 g, pantothenic acid 40 g, niacin 80 g, folic acid 1.5 g, biotin 0.2 g
^cNutrient levels were calculated values, which based on the formula data of the basal diet

ISSN: 2049-1891