Table 2 Primer sequences for qRT-PCR
Gene | Primer | Primer sequence (5'→3') | Accession No. |
|---|---|---|---|
β-Actin | Forward | TGCGTGACATCAAGGAGAAG | NM_205518 |
Reverse | TGCCAGGGTACATTGTGGTA | ||
AMPK | Forward | ATCTGTCTCGCCCTCATCCT | NM_001039603.2 |
Reverse | CCACTTCGCTCTTCTTACACCTT | ||
ACACA | Forward | CCGAGAACCCAAAACTACCAG | NM_205505.1 |
Reverse | GCCAGCAGTCTGAGCCACTA | ||
FASN | Forward | AAAGCAATTCGTCACGGACA | NM_205155.4 |
Reverse | GGCACCATCAGGACTAAGCA | ||
PPARA | Forward | AGGCCAAGTTGAAAGCAGAA | NM_001001464.1 |
Reverse | TTTCCCTGCAAGGATGACTC | ||
CD36 | Forward | CTGGGAAGGTTACTGCGATT | NM_001030731.1 |
Reverse | GCGAGGAACTGTGAAACGATA | ||
SREBP1c | Forward | GCCCTCTGTGCCTTTGTCTTC | NM_204126.2 |
Reverse | ACTCAGCCATGATGCTTCTTC | ||
SCD1 | Forward | AGCAGAACGAGGCATGGTAG | NM_204890.2 |
Reverse | GGATCAGCGTCAGCCCAATA | ||
PPARG | Forward | TGACAGGAAAGACGACAGACA | NM_001001460.2 |
Reverse | CTCCACAGAGCGAAACTGAC | ||
CPT1A | Forward | TCGTCTTGCCATGACTGGTG | XM_040700878.2 |
Reverse | GCTGTGGTGTCTGACTCGTT | ||
HMGCR | Forward | CATAGGTGGCTACAACG | NM_204485.3 |
Reverse | TACGCTCCATCAAAGTG | ||
ChREBP | Forward | GATGAGCACCGCAAACCAGAGG | NM_001398177.1 |
Reverse | TCGGAGCCGCTTCTTGTAGTAGG | ||
LXRα | Forward | CAAAGGGAATGAATGAGC | NM_204542.3 |
Reverse | AGCCGAAGGGCAAACAC | ||
FXR | Forward | AGTAGAAGCCATGTTCCTCCGTT | NM_001396910.1 |
Reverse | GCAGTGCATATTCCTCCTGTGTC | ||
ACOX1 | Forward | ATGTCACGTTCACCCCATCC | NM_001006205.2 |
Reverse | AGGTAGGAGACCATGCCAGT | ||
SIRT1 | Forward | TAGCCAATGGTTTCCACTCC | NM_001004767.2 |
Reverse | AAGAATTGTCCGTGGGTCTG | ||
FABP3 | Forward | TCTGAAAGCTCTTGCACTGCC | NM_001030889.2 |
Reverse | TCACAGTCTGCCTGGGTGTT | ||
FABP4 | Forward | GCACCTGGAAGCTCCTTTCT | NM_204290.2 |
Reverse | ATTAGGCTTGGCCACACCAG | ||
SCP2 | Forward | AGGAGGCAACCTGGGTAGT | NM_001305200.2 |
Reverse | ATTTGCCTTGAAAGAAGGCTGTC | ||
ABCA1 | Forward | TCAATCACCCGCTCAACT | NM_204145.3 |
Reverse | CTGGCAGGAACAAAGGAC |