Caffeic acid and chlorogenic acid mediate the ADPN-AMPK-PPARα pathway to improve fatty liver and production performance in laying hens

Tian, Wenjie; Gonzales, Gerard Bryan; Wang, Hao; Yang, Youyou; Tang, Chaohua; Zhao, Qingyu; Zhang, Junmin; Zhang, Huiyan; Qin, Yuchang

doi:10.1186/s40104-025-01175-z

Journal of Animal Science and Biotechnology

Table 5 Effect of the dietary CA and CGA on the routine blood test

From: Caffeic acid and chlorogenic acid mediate the ADPN-AMPK-PPARα pathway to improve fatty liver and production performance in laying hens

Item	CON	CA25	CA50	CA100	CA200	P-value	CON	CGA100	CGA200	CGA400	P-value
WBC	117.75 ± 0.50^a	114.02 ± 0.86^b	113.36 ± 1.20^b	116.39 ± 0.4^ab	113.61 ± 1.65^b	0.004	117.75 ± 0.50^a	114.16 ± 0.96^b	113.78 ± 0.826^b	111.76 ± 1.026^b	< 0.001
LYM#	46.78 ± 1.51^b	49.05 ± 0.81^ab	51.64 ± 1.11^a	46.37 ± 0.65^b	48.27 ± 1.22^b	< 0.001	46.78 ± 1.51	47.50 ± 0.85	47.55 ± 1.12	48.43 ± 1.20	0.654
MID#	16.88 ± 0.61^ab	16.85 ± 0.11^ab	16.99 ± 0.11^a	16.64 ± 0.14^ab	16.52 ± 0.17^b	0.048	16.88 ± 0.61^a	16.53 ± 0.08^b	16.58 ± 0.09^b	16.41 ± 0.06^b	< 0.001
GRA#	54.09 ± 0.91^a	48.11 ± 1.56^ab	44.73 ± 2.21^b	53.38 ± 1.01^a	48.83 ± 2.67^ab	< 0.001	54.09 ± 0.91^a	50.13 ± 1.7^ab	49.65 ± 1.98^ab	46.92 ± 2.20^b	0.039
LYM%	39.83 ± 0.54^b	43.20 ± 0.98^ab	45.78 ± 1.39^a	39.94 ± 0.63^b	42.77 ± 1.65^ab	< 0.001	39.83 ± 0.54	41.80 ± 1.10	41.98 ± 1.30	43.58 ± 1.48	0.140
MID%	14.29 ± 0.08^b	14.73 ± 0.13^ab	14.94 ± 0.14^a	14.27 ± 0.15^b	14.50 ± 0.17^ab	0.002	14.29 ± 0.08	14.43 ± 0.12	14.53 ± 0.17	14.64 ± 0.15	0.265
GRA%	45.88 ± 0.62^a	42.07 ± 1.10^ab	39.28 ± 1.52^b	45.79 ± 0.76^a	42.73 ± 1.79^ab	< 0.001	45.88 ± 0.62	43.77 ± 1.20	43.48 ± 1.45	41.78 ± 1.62	0.146
RBC	3.00 ± 0.06	2.97 ± 0.08	3.00 ± 0.11	2.96 ± 0.05	3.10 ± 0.08	0.669	3.00 ± 0.06	2.90 ± 0.08	3.03 ± 0.07	2.97 ± 0.09	0.659
HGB	116.25 ± 2.07	114.67 ± 3.82	116.90 ± 4.19	114.45 ± 2.51	121.60 ± 2.40	0.468	116.25 ± 2.07^a	113.17 ± 3.45^a	63.75 ± 6.69^b	50.17 ± 2.81^b	< 0.001
HCT	0.28 ± 0.01	0.28 ± 0.01	0.28 ± 0.01	0.27 ± 0.00	0.29 ± 0.01	0.597	0.28 ± 0.01	0.27 ± 0.01	0.29 ± 0.01	0.28 ± 0.01	0.319
MCV	92.98 ± 0.63	94.67 ± 1.18	92.36 ± 0.91	93.02 ± 0.9	93.02 ± 1.16	0.474	92.98 ± 0.63	94.81 ± 0.92	95.89 ± 1.04	93.18 ± 1.10	0.084
MCH	38.71 ± 0.29	38.54 ± 0.58	38.87 ± 0.54	38.59 ± 0.40	39.61 ± 0.48	0.499	38.71 ± 0.29^a	38.83 ± 0.38^a	21.33 ± 2.66^b	16.69 ± 0.57^b	< 0.001
MCHC	395.33 ± 3.01	386.83 ± 6.13	399.50 ± 4.15	394.09 ± 4.07	400.10 ± 4.60	0.208	395.33 ± 3.01^a	389.25 ± 5.56^a	211.17 ± 26.16^b	170.50 ± 6.28^b	< 0.001
RDW-SD	17.10 ± 3.13	24.98 ± 4.41	20.85 ± 3.96	21.21 ± 3.74	28.25 ± 3.75	0.261	17.10 ± 3.13	27.77 ± 3.40	25.22 ± 3.48	23.80 ± 3.42	0.128
RDW-CV	29.58 ± 0.58	28.99 ± 0.50	29.17 ± 0.93	29.29 ± 0.88	28.86 ± 0.89	0.961	29.58 ± 0.58	29.58 ± 0.56	30.64 ± 0.97	30.28 ± 0.56	0.592
PLT	21.00 ± 2.46^b	31.18 ± 2.49^ab	28.56 ± 5.88^b	27.18 ± 2.62^b	44.75 ± 6.67^a	0.002	21.00 ± 2.46	28.00 ± 3.02	31.64 ± 3.46	33.36 ± 6.76	0.179
PCT	0.02 ± 0.00	0.03 ± 0.00	0.05 ± 0.02	0.03 ± 0.00	0.04 ± 0.01	0.184	0.02 ± 0.00	0.03 ± 0.00	0.04 ± 0.01	0.03 ± 0.01	0.089
MPV	0.97 ± 0.06	11.00 ± 0.09	10.68 ± 0.19	10.87 ± 0.07	10.88 ± 0.11	0.237	10.97 ± 0.06^ab	10.91 ± 0.07^ab	11.14 ± 0.07^a	10.78 ± 0.13^b	0.036
PDW	77.75 ± 5.15^a	63.38 ± 3.57^abc	59.03 ± 6.28^bc	69.19 ± 5.16^ab	50.66 ± 3.89^c	0.002	77.75 ± 5.15^a	65.73 ± 4.86^ab	55.31 ± 6.17^b	72.41 ± 5.42^ab	0.024

WBC White blood cells count, LYM# Lymphocyte absolute value, MID# Middle cell absolute value, GRA# Granulocyte absolute value, LYM% Lymphocyte percentage, MID% Middle cell percentage, GRA% Granulocyte percentage, RBC Red blood cells count, HGB Hemoglobin, HCT Hematocrit, MCV Mean corpuscular volume, MCH Mean corpuscular hemoglobin, MCHC Mean corpuscular hemoglobin concentration, RDW-SD Red cell distribution width of standard deviation, RDW-CV Red cell distribution width coefficient of variation, PLT Platelets, PCT Platelet crit, MPV Mean platelet volume, PDW Platelet distribution width
The assessment measures were conducted at week 12. All data are presented as mean ± SEM (n = 10)
^a−cMeans with different superscripts within the groups differ significantly (P < 0.05)

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ISSN: 2049-1891

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