Fig. 3

Methionine-rescue regulates Wnt/β-catenin pathway and mitochondrial function. A Pearson correlation analysis of whole genes in control group, methionine-deficiency group and methionine-rescue group (n = 4). B Principal component analysis of genes in control group, methionine deficiency group and methionine-rescue group samples (n = 4). C and D GO enrichment analysis (C) and KEGG enrichment analysis (D) of differential genes in dorsal skin tissue of broiler chickens between control group and Met-deficiency group. E and F GO enrichment analysis (E) and KEGG enrichment analysis (F) of differential genes in dorsal skin tissue of broiler chickens between Met-deficiency group and Met-rescue group. G and I Methionine deficiency (28 d) on the expression of key proteins of Wnt/β-catenin (G) and mitochondria related proteins (I) in the dorsal skin of broiler chickens. H and J Methionine deficiency is followed by dietary methionine supplementation, and its influences in key proteins of Wnt/β-catenin (H) and mitochondria related proteins (J) in the dorsal skin of broiler chickens. Control, Control group; Met-deficiency, Methionine deficiency group; Met-rescue, Methionine supplement group; Axin1, Axis inhibition protein 1; TCF4, Transcription factor 4; COX IV, Cytochrome c oxidase subunit IV; PGAM5, Phosphoglycerate mutase 5