Fig. 6
FPM reduced oxidative stress but did not improve intestinal barrier integrity in C. elegans and D. melanogaster. (a) C. elegans activity assay with FPM treatment and oxidative stress using PQ. Three independent experiments with eight wells (n = 24). (b) Activity counts over time were summarized with the AUC to represent the antioxidant capacity. Three independent experiments with eight wells (n = 24). (c) Smurf assay for intestinal barrier investigation in C. elegans with FPM treatment. Three independent experiments (n = 3). (d) ROS assay in D. melanogaster with FPM treatment and oxidative stress using FeSO4. Fluorescence represents ROS levels. Three independent experiments in triplicates (n = 9). (e) Mortality analysis in D. melanogaster with FPM treatment and intestinal stress using DSS. Three independent experiments with 3-4 replicates (n = 10). (f) Smurf assay for intestinal barrier investigation in D. melanogaster with FPM treatment and intestinal stress using DSS. Three independent experiments with 3–4 replicates (n = 10). ****P < 0.0001, *P < 0.05 and ns P ≥ 0.05, compared to oxidative stress control (b, d, e, f) or untreated control (c) (one-way ANOVA)