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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: ROS-DRP1-mediated excessive mitochondrial fission and autophagic flux inhibition contribute to heat stress-induced apoptosis in goat Sertoli cells

Fig. 5

HS induces mitochondrial dysfunction and apoptosis in SCs. A and B Mitochondrial Ca2+ levels in SCs after HS exposure. C Protein expression levels of the MCU in SCs after HS exposure. D and E JC-1 staining was employed to assess the effect of HS on mitochondrial membrane potential. F ATP levels in SCs were measured using luciferase-based assays. G Expression of proteins involved in mitochondria-dependent apoptosis. H–J Quantification of proteins using Image J. *P < 0.05, **P < 0.01 vs. control

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