Fig. 9

HS-induced mitochondrial fragmentation is associated with the inhibition of mitophagy flux. A and B Western blotting images and associated quantitative evaluations of autophagy markers in SCs. C and D Western blotting images and quantitative analyses of Ub expression in SCs after HS exposure. E and F Representative images and analysis of mitotracker (red) and lysotracker (green) colocalization in SCs using confocal microscopy. Scale bar = 20 μm. Blue: DAPI staining. *P < 0.05, **P < 0.01 vs. control; # P < 0.05 vs. HS