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Fig. 7 | Journal of Animal Science and Biotechnology

Fig. 7

From: Lactate induces oxidative stress by HIF1α stabilization and circadian clock disturbance in mammary gland of dairy cows

Fig. 7

Stabilized HIF1α binds to the HRE sequence to regulate oxidative stress and circadian. a Luciferase activity driven by promoters containing HREs with different concentration of sodium lactate. b The knock-down efficiency of HIF1α was verified by qPCR. c The promoter of oxidative stress and circadian related genes contain HRE elements. d ChIP-qPCR analysis of BMAL1, HMOX1 and FOXO3 binding to HIF1α promoter. n = 3 per condition. e The protein levels of BMAL1, CLOCK, HMOX1 and FOXO3 after knocking down HIF1α. f Graphical abstract of lactate induced oxidative stress of mammary gland and disturbance of mammary gland circadian by stabilizing HIF1α

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891