Fig. 7

Involvement of IFI44 gene in the regulation of PBMC apoptosis and proliferation induced by Poly(I:C) challenge. A Workflow of IFI44 gene function validation. B The relative expression of IFI44 gene after 24 h transfection with small interfering RNA. C The relative expression level of IFI44 gene stimulated by Poly(I:C) for 24 h. D The interference with the IFI44 gene regulated the cell apoptosis, necrosis and cell proliferation induced by Poly(I:C). E Cell apoptosis and necrosis was assessed with fluorescence microscopy. Apoptotic cells were stained green by YO-PRO-1, and necrosis cells were stained red by PI (Propidium Iodide). siRNA-IFI44: IFI44 gene interference group. siRNA-NC: negative control. siRNA-IFI44 + Poly IC: After interfering IFI44 gene for 24 h, the treatment group was challenged with Poly(I:C). siRNA-NC + Poly IC: The negative control group was challenged with Poly(I:C) after 24 h